Gellan lyase (EC 4.2.2.25) is an enzyme with systematic name gellan β-D-glucopyranosyl-(1→4)-D-glucopyranosyluronate lyase.[1][2][3] This enzyme catalyses the following process:

Gellan lyase
Identifiers
EC no.4.2.2.25
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
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NCBIproteins
Eliminative cleavage of β-D-glucopyranosyl-(1→4)-β-D-glucopyranosyluronate bonds of gellan backbone releasing tetrasaccharides containing a 4-deoxy-4,5-unsaturated D-glucopyranosyluronic acid at the non-reducing end. The tetrasaccharide produced from deacetylated gellan is β-D-4-deoxy-Δ4-GlcAp-(1→4)-β-DGlcp-(1→4)-α-L-Rhap-(1→3)-β-D-Glcp.

References

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  1. ^ Hashimoto W, Maesaka K, Sato N, Kimura S, Yamamoto K, Kumagai H, Murata K (March 1997). "Microbial system for polysaccharide depolymerization: enzymatic route for gellan depolymerization by Bacillus sp. GL1". Archives of Biochemistry and Biophysics. 339 (1): 17–23. doi:10.1006/abbi.1996.9851. PMID 9056228.17-23&rft.date=1997-03&rft_id=info:doi/10.1006/abbi.1996.9851&rft_id=info:pmid/9056228&rft.aulast=Hashimoto&rft.aufirst=W&rft.au=Maesaka, K&rft.au=Sato, N&rft.au=Kimura, S&rft.au=Yamamoto, K&rft.au=Kumagai, H&rft.au=Murata, K&rfr_id=info:sid/en.wikipedia.org:Gellan lyase" class="Z3988">
  2. ^ Hashimoto W, Sato N, Kimura S, Murata K (June 1998). "Polysaccharide lyase: molecular cloning of gellan lyase gene and formation of the lyase from a huge precursor protein in Bacillus sp. GL1". Archives of Biochemistry and Biophysics. 354 (1): 31–9. doi:10.1006/abbi.1998.0674. PMID 9633595.31-9&rft.date=1998-06&rft_id=info:doi/10.1006/abbi.1998.0674&rft_id=info:pmid/9633595&rft.aulast=Hashimoto&rft.aufirst=W&rft.au=Sato, N&rft.au=Kimura, S&rft.au=Murata, K&rfr_id=info:sid/en.wikipedia.org:Gellan lyase" class="Z3988">
  3. ^ Miyake O, Kobayashi E, Nankai H, Hashimoto W, Mikami B, Murata K (February 2004). "Posttranslational processing of polysaccharide lyase: maturation route for gellan lyase in Bacillus sp. GL1". Archives of Biochemistry and Biophysics. 422 (2): 211–20. doi:10.1016/j.abb.2003.12.015. PMID 14759609.211-20&rft.date=2004-02&rft_id=info:doi/10.1016/j.abb.2003.12.015&rft_id=info:pmid/14759609&rft.aulast=Miyake&rft.aufirst=O&rft.au=Kobayashi, E&rft.au=Nankai, H&rft.au=Hashimoto, W&rft.au=Mikami, B&rft.au=Murata, K&rfr_id=info:sid/en.wikipedia.org:Gellan lyase" class="Z3988">
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