DAB2

Disabled homolog 2 is a protein that in humans is encoded by the DAB2 gene.

DAB2
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2LSW
Identifiers
Aliases	DAB2, DOC-2, DOC2, clathrin adaptor protein, DAB adaptor protein 2
External IDs	OMIM: 601236; MGI: 109175; HomoloGene: 1026; GeneCards: DAB2; OMA:DAB2 - orthologs
Gene location (Human) Chr. Chromosome 5 (human) Band 5p13.1 Start 39,371,675 bp End 39,462,300 bp
Gene location (Mouse) Chr. Chromosome 15 (mouse) Band 15 A1|15 2.15 cM Start 6,329,269 bp End 6,470,193 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in caput epididymis corpus epididymis tendon of biceps brachii placenta synovial joint Achilles tendon right adrenal cortex human kidney synovial membrane left adrenal gland Top expressed in yolk sac stroma of bone marrow efferent ductule right kidney zygote human kidney dermis adrenal gland proximal tubule secondary oocyte More reference expression data BioGPS More reference expression data
Gene ontology Molecular function clathrin adaptor activity protein C-terminus binding protein binding cargo receptor activity SMAD binding Cellular component cytoplasm intracellular membrane-bounded organelle membrane focal adhesion plasma membrane lysosomal membrane nucleolus clathrin-coated vesicle membrane clathrin-coated pit extracellular exosome cytoplasmic vesicle clathrin-coated vesicle fibrillar center cytosol Biological process positive regulation of transforming growth factor beta receptor signaling pathway cell differentiation positive regulation of protein phosphorylation endocytosis positive regulation of cell migration positive regulation of pathway-restricted SMAD protein phosphorylation negative regulation of androgen receptor signaling pathway negative regulation of apoptotic process Wnt signaling pathway negative regulation of protein binding positive regulation of epithelial to mesenchymal transition leading edge cell differentiation multicellular organism development positive regulation of transcription, DNA-templated positive regulation of endocytosis negative regulation of protein localization to plasma membrane positive regulation of clathrin-dependent endocytosis protein transport positive regulation of early endosome to late endosome transport integrin-mediated signaling pathway positive regulation of Wnt signaling pathway, planar cell polarity pathway cell population proliferation negative regulation of transcription, DNA-templated negative regulation of canonical Wnt signaling pathway positive regulation of proteasomal ubiquitin-dependent protein catabolic process positive regulation of SMAD protein signal transduction apoptotic process receptor-mediated endocytosis membrane organization transport Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 1601 13132 Ensembl ENSG00000153071 ENSMUSG00000022150 UniProt P98082 P98078 RefSeq (mRNA) NM_001343 NM_001244871 NM_001008702 NM_001037905 NM_001102400 NM_023118 NM_001310446 RefSeq (protein) NP_001231800 NP_001334 NP_001008702 NP_001032994 NP_001095870 NP_001297375 NP_075607 Location (UCSC) Chr 5: 39.37 – 39.46 Mb Chr 15: 6.33 – 6.47 Mb PubMed search
Wikidata
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Function

DAB2 mRNA is expressed in normal ovarian epithelial cells but is down-regulated or absent from ovarian carcinoma cell lines. The 770-amino acid predicted protein has an overall 83% identity with the mouse p96 protein, a putative mitogen-responsive phosphoprotein; homology is strongest in the amino-terminal end of the protein in a region corresponding to the phosphotyrosine interaction domain. The down-regulation of DAB2 may play an important role in ovarian carcinogenesis. This gene was initially named DOC2 (for Differentially expressed in Ovarian Cancer) and is distinct from the DOC2A and DOC2B genes (for double C2-like domains, alpha and beta).

Interactions

DAB2 has been shown to interact with:

• C-src tyrosine kinase,
• Cdk1,
• DAB2IP,
• DVL2,
• DVL3,
• LRP2,
• MYO6,
• Mothers against decapentaplegic homolog 2,
• Mothers against decapentaplegic homolog 3
• PIN1, and
• Src.

Further reading

• Mok SC, Wong KK, Chan RK, Lau CC, Tsao SW, Knapp RC, Berkowitz RS (1994). "Molecular cloning of differentially expressed genes in human epithelial ovarian cancer". Gynecol. Oncol. 52 (2): 247–52. doi:10.1006/gyno.1994.1040. PMID 8314147.
• Bonaldo MF, Lennon G, Soares MB (1996). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Res. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
• Xu XX, Yi T, Tang B, Lambeth JD (1998). "Disabled-2 (Dab2) is an SH3 domain-binding partner of Grb2". Oncogene. 16 (12): 1561–9. doi:10.1038/sj.onc.1201678. PMID 9569023.
• Fazili Z, Sun W, Mittelstaedt S, Cohen C, Xu XX (1999). "Disabled-2 inactivation is an early step in ovarian tumorigenicity". Oncogene. 18 (20): 3104–13. doi:10.1038/sj.onc.1202649. PMID 10340382.
• Tseng CP, Ely BD, Pong RC, Wang Z, Zhou J, Hsieh JT (1999). "The role of DOC-2/DAB2 protein phosphorylation in the inhibition of AP-1 activity. An underlying mechanism of its tumor-suppressive function in prostate cancer". J. Biol. Chem. 274 (45): 31981–6. doi:10.1074/jbc.274.45.31981. PMID 10542228.
• Oleinikov AV, Zhao J, Makker SP (2000). "Cytosolic adaptor protein Dab2 is an intracellular ligand of endocytic receptor gp600/megalin". Biochem. J. 347 Pt 3 (3): 613–21. doi:10.1042/0264-6021:3470613. PMC 1220996. PMID 10769163.
• Sheng Z, Sun W, Smith E, Cohen C, Sheng Z, Xu XX (2000). "Restoration of positioning control following Disabled-2 expression in ovarian and breast tumor cells". Oncogene. 19 (42): 4847–54. doi:10.1038/sj.onc.1203853. PMID 11039902.
• Sheng Z, He J, Tuppen JA, Sun W, Fazili Z, Smith ER, Dong FB, Xu XX (2000). "Structure, sequence, and promoter analysis of human disabled-2 gene (DAB2)". Genomics. 70 (3): 381–6. doi:10.1006/geno.2000.6383. PMID 11161789.
• Morris SM, Cooper JA (2001). "Disabled-2 colocalizes with the LDLR in clathrin-coated pits and interacts with AP-2". Traffic. 2 (2): 111–23. doi:10.1034/j.1600-0854.2001.020206.x. PMID 11247302. S2CID 33186168.
• Zhou J, Hsieh JT (2001). "The inhibitory role of DOC-2/DAB2 in growth factor receptor-mediated signal cascade. DOC-2/DAB2-mediated inhibition of ERK phosphorylation via binding to Grb2". J. Biol. Chem. 276 (30): 27793–8. doi:10.1074/jbc.M102803200. PMID 11371563.
• Hocevar BA, Smine A, Xu XX, Howe PH (2001). "The adaptor molecule Disabled-2 links the transforming growth factor beta receptors to the Smad pathway". EMBO J. 20 (11): 2789–801. doi:10.1093/emboj/20.11.2789. PMC 125498. PMID 11387212.
• Wang Z, Tseng CP, Pong RC, Chen H, McConnell JD, Navone N, Hsieh JT (2002). "The mechanism of growth-inhibitory effect of DOC-2/DAB2 in prostate cancer. Characterization of a novel GTPase-activating protein associated with N-terminal domain of DOC-2/DAB2". J. Biol. Chem. 277 (15): 12622–31. doi:10.1074/jbc.M110568200. PMID 11812785.
• Inoue A, Sato O, Homma K, Ikebe M (2002). "DOC-2/DAB2 is the binding partner of myosin VI". Biochem. Biophys. Res. Commun. 292 (2): 300–7. doi:10.1006/bbrc.2002.6636. PMID 11906161.
• Mishra SK, Keyel PA, Hawryluk MJ, Agostinelli NR, Watkins SC, Traub LM (2002). "Disabled-2 exhibits the properties of a cargo-selective endocytic clathrin adaptor". EMBO J. 21 (18): 4915–26. doi:10.1093/emboj/cdf487. PMC 126284. PMID 12234931.
• Morris SM, Tallquist MD, Rock CO, Cooper JA (2002). "Dual roles for the Dab2 adaptor protein in embryonic development and kidney transport". EMBO J. 21 (7): 1555–64. doi:10.1093/emboj/21.7.1555. PMC 125955. PMID 11927540.
• Morris SM, Arden SD, Roberts RC, Kendrick-Jones J, Cooper JA, Luzio JP, Buss F (2002). "Myosin VI binds to and localises with Dab2, potentially linking receptor-mediated endocytosis and the actin cytoskeleton". Traffic. 3 (5): 331–41. doi:10.1034/j.1600-0854.2002.30503.x. PMID 11967127. S2CID 25079713.
• Zhou J, Scholes J, Hsieh JT (2003). "Characterization of a novel negative regulator (DOC-2/DAB2) of c-Src in normal prostatic epithelium and cancer". J. Biol. Chem. 278 (9): 6936–41. doi:10.1074/jbc.M210628200. PMID 12473651.
• Calderwood DA, Fujioka Y, de Pereda JM, García-Alvarez B, Nakamoto T, Margolis B, McGlade CJ, Liddington RC, Ginsberg MH (2003). "Integrin beta cytoplasmic domain interactions with phosphotyrosine-binding domains: a structural prototype for diversity in integrin signaling". Proc. Natl. Acad. Sci. U.S.A. 100 (5): 2272–7. Bibcode:2003PNAS..100.2272C. doi:10.1073/pnas.262791999. PMC 151330. PMID 12606711.