5β-Reductase, or Δ4-3-oxosteroid 5β-reductase (EC 1.3.1.3, 3-oxo-Δ4-steroid 5β-reductase, androstenedione 5β-reductase, cholestenone 5β-reductase, cortisone 5β-reductase, cortisone Δ4-5β-reductase, steroid 5β-reductase, testosterone 5β-reductase, Δ4-3-ketosteroid 5β-reductase, Δ4-5β-reductase, Δ4-hydrogenase, 4,5β-dihydrocortisone:NADP Δ4-oxidoreductase, 3-oxo-5β-steroid:NADP Δ4-oxidoreductase) is an enzyme with systematic name 5β-cholestan-3-one:NADP 4,5-oxidoreductase.[1][2][3][4][5][6][7][8][9]
| Δ4-3-Oxosteroid 5β-reductase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.3.1.3 | ||||||||
| CAS no. | 2604034 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
This enzyme catalyses the following chemical reaction:
- (1) 5β-cholestan-3-one NADP cholest-4-en-3-one NADPH H
- (2) 17,21-dihydroxy-5β-pregnane-3,11,20-trione NADP cortisone NADPH H
The human enzyme efficiently acts on progesterone, 17α-hydroxyprogesterone, androstenedione, and testosterone to transform them into 5β-reduced metabolites. It can also act on aldosterone, corticosterone, and cortisol.
See also
editReferences
edit- ^ Dorfman RI, Forchielli E (November 1956). "Separation of delta 4-5 alpha-hydrogenases from rat liver homogenates". The Journal of Biological Chemistry. 223 (1): 443–8. PMID 13376613.443-8&rft.date=1956-11&rft_id=info:pmid/13376613&rft.aulast=Dorfman&rft.aufirst=RI&rft.au=Forchielli, E&rfr_id=info:sid/en.wikipedia.org:5β-Reductase" class="Z3988">
- ^ Brown-Grant K, Forchielli E, Dorfman RI (May 1960). "The delta4-hydrogenases of guinea pig adrenal gland". The Journal of Biological Chemistry. 235: 1317–20. PMID 13805063.1317-20&rft.date=1960-05&rft_id=info:pmid/13805063&rft.aulast=Brown-Grant&rft.aufirst=K&rft.au=Forchielli, E&rft.au=Dorfman, RI&rfr_id=info:sid/en.wikipedia.org:5β-Reductase" class="Z3988">
- ^ Levy, H.R. & Talalay, P. (1957). "Enzymatic introduction of double bonds into steroid ring A". J. Am. Chem. Soc. 79 (10): 2658–2659. doi:10.1021/ja01567a089.2658-2659&rft.date=1957&rft_id=info:doi/10.1021/ja01567a089&rft.au=Levy, H.R.&rft.au=Talalay, P.&rfr_id=info:sid/en.wikipedia.org:5β-Reductase" class="Z3988">
- ^ Tomkins GM (March 1957). "The enzymatic reduction of delta 4-3-ketosteroids". The Journal of Biological Chemistry. 225 (1): 13–24. PMID 13416214.13-24&rft.date=1957-03&rft_id=info:pmid/13416214&rft.aulast=Tomkins&rft.aufirst=GM&rfr_id=info:sid/en.wikipedia.org:5β-Reductase" class="Z3988">
- ^ Sugimoto Y, Yoshida M, Tamaoki B (December 1990). "Purification of 5 beta-reductase from hepatic cytosol fraction of chicken". The Journal of Steroid Biochemistry and Molecular Biology. 37 (5): 717–24. doi:10.1016/0960-0760(90)90356-p. PMID 2278855. S2CID 54431282.717-24&rft.date=1990-12&rft_id=https://api.semanticscholar.org/CorpusID:54431282#id-name=S2CID&rft_id=info:pmid/2278855&rft_id=info:doi/10.1016/0960-0760(90)90356-p&rft.aulast=Sugimoto&rft.aufirst=Y&rft.au=Yoshida, M&rft.au=Tamaoki, B&rfr_id=info:sid/en.wikipedia.org:5β-Reductase" class="Z3988">
- ^ Furuebisu M, Deguchi S, Okuda K (March 1987). "Identification of cortisone 5 beta-reductase as delta 4-3-ketosteroid 5 beta-reductase". Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. 912 (1): 110–4. doi:10.1016/0167-4838(87)90253-6. PMID 3828348.110-4&rft.date=1987-03&rft_id=info:doi/10.1016/0167-4838(87)90253-6&rft_id=info:pmid/3828348&rft.aulast=Furuebisu&rft.aufirst=M&rft.au=Deguchi, S&rft.au=Okuda, K&rfr_id=info:sid/en.wikipedia.org:5β-Reductase" class="Z3988">
- ^ Okuda A, Okuda K (June 1984). "Purification and characterization of delta 4-3-ketosteroid 5 beta-reductase". The Journal of Biological Chemistry. 259 (12): 7519–24. PMID 6736016.7519-24&rft.date=1984-06&rft_id=info:pmid/6736016&rft.aulast=Okuda&rft.aufirst=A&rft.au=Okuda, K&rfr_id=info:sid/en.wikipedia.org:5β-Reductase" class="Z3988">
- ^ Charbonneau A, The VL (January 2001). "Genomic organization of a human 5beta-reductase and its pseudogene and substrate selectivity of the expressed enzyme". Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 1517 (2): 228–35. doi:10.1016/s0167-4781(00)00278-5. PMID 11342103.228-35&rft.date=2001-01&rft_id=info:doi/10.1016/s0167-4781(00)00278-5&rft_id=info:pmid/11342103&rft.aulast=Charbonneau&rft.aufirst=A&rft.au=The, VL&rfr_id=info:sid/en.wikipedia.org:5β-Reductase" class="Z3988">
- ^ Kondo KH, Kai MH, Setoguchi Y, Eggertsen G, Sjöblom P, Setoguchi T, Okuda KI, Björkhem I (January 1994). "Cloning and expression of cDNA of human delta 4-3-oxosteroid 5 beta-reductase and substrate specificity of the expressed enzyme". European Journal of Biochemistry. 219 (1–2): 357–63. doi:10.1111/j.1432-1033.1994.tb19947.x. PMID 7508385.1–2&rft.pages=357-63&rft.date=1994-01&rft_id=info:doi/10.1111/j.1432-1033.1994.tb19947.x&rft_id=info:pmid/7508385&rft.aulast=Kondo&rft.aufirst=KH&rft.au=Kai, MH&rft.au=Setoguchi, Y&rft.au=Eggertsen, G&rft.au=Sjöblom, P&rft.au=Setoguchi, T&rft.au=Okuda, KI&rft.au=Björkhem, I&rft_id=https://doi.org/10.1111%2Fj.1432-1033.1994.tb19947.x&rfr_id=info:sid/en.wikipedia.org:5β-Reductase" class="Z3988">
External links
edit- Delta4-3-oxosteroid 5beta-reductase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
