In enzymology, a 3alpha(or 20beta)-hydroxysteroid dehydrogenase (EC 1.1.1.53) is an enzyme that catalyzes the chemical reaction
| 3-alpha(or 20-beta)-hydroxysteroid dehydrogenase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 3-alpha,20-beta-hydroxysteroid dehydrogenase tetramer, Streptomyces exfoliatus | |||||||||
| Identifiers | |||||||||
| EC no. | 1.1.1.53 | ||||||||
| CAS no. | 9028-42-6 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
- androstan-3alpha,17beta-diol NAD 17beta-hydroxyandrostan-3-one NADH H
Thus, the two substrates of this enzyme are androstan-3alpha,17beta-diol and NAD , whereas its 3 products are 17beta-hydroxyandrostan-3-one, NADH, and H .
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD or NADP as acceptor. The systematic name of this enzyme class is 3alpha(or 20beta)-hydroxysteroid:NAD oxidoreductase. Other names in common use include cortisone reductase, (R)-20-hydroxysteroid dehydrogenase, dehydrogenase, 20beta-hydroxy steroid, Delta4-3-ketosteroid hydrogenase, 20beta-hydroxysteroid dehydrogenase, 3alpha,20beta-hydroxysteroid:NAD -oxidoreductase, NADH-20beta-hydroxysteroid dehydrogenase, and 20beta-HSD. This enzyme participates in bile acid biosynthesis and c21-steroid hormone metabolism.
Structural studies
editAs of late 2007, 6 structures have been solved for this class of enzymes, with PDB accession codes 1HDC, 1N5D, 1NFF, 1NFQ, 1NFR, and 2HSD.
References
edit- Edwards CA, Orr JC (1978). "Comparison of the 3α- and 20β-Hydroxysteroid Dehydrogenase Activities of the Cortisone Reductase of Streptomyces hydrogenans". Biochemistry. 17 (21): 4370–6. doi:10.1021/bi00614a003. PMID 718844.4370-6&rft.date=1978&rft_id=info:doi/10.1021/bi00614a003&rft_id=info:pmid/718844&rft.aulast=Edwards&rft.aufirst=CA&rft.au=Orr, JC&rfr_id=info:sid/en.wikipedia.org:3alpha(or 20beta)-hydroxysteroid dehydrogenase" class="Z3988">
- Kung CC, Huang WN, Huang YC, Yeh KC (2006). "Proteomic survey of copper-binding proteins in Arabidopsis roots by immobilized metal affinity chromatography and mass spectrometry". Proteomics. 6 (9): 2746–58. doi:10.1002/pmic.200500108. PMID 16526091. S2CID 25896917.2746-58&rft.date=2006&rft_id=https://api.semanticscholar.org/CorpusID:25896917#id-name=S2CID&rft_id=info:pmid/16526091&rft_id=info:doi/10.1002/pmic.200500108&rft.aulast=Kung&rft.aufirst=CC&rft.au=Huang, WN&rft.au=Huang, YC&rft.au=Yeh, KC&rfr_id=info:sid/en.wikipedia.org:3alpha(or 20beta)-hydroxysteroid dehydrogenase" class="Z3988">
- Lynn WS Jr, Brown RH (1958). "The conversion of progesterone to androgens by testes". J. Biol. Chem. 232 (2): 1015–30. doi:10.1016/S0021-9258(19)77419-5. PMID 13549484.1015-30&rft.date=1958&rft_id=info:doi/10.1016/S0021-9258(19)77419-5&rft_id=info:pmid/13549484&rft.au=Lynn WS Jr&rft.au=Brown, RH&rft_id=https://doi.org/10.1016%2FS0021-9258%2819%2977419-5&rfr_id=info:sid/en.wikipedia.org:3alpha(or 20beta)-hydroxysteroid dehydrogenase" class="Z3988">
- Strickler RC, Covey DF, Tobias B (1980). "Study of 3α,20β-Hydroxysteroid Dehydrogenase with an Enzyme-Generated Affinity Alkylator: Dual Enzyme Activity at a Single Active Site". Biochemistry. 19 (22): 4950–4. doi:10.1021/bi00563a002. PMID 6936053.4950-4&rft.date=1980&rft_id=info:doi/10.1021/bi00563a002&rft_id=info:pmid/6936053&rft.aulast=Strickler&rft.aufirst=RC&rft.au=Covey, DF&rft.au=Tobias, B&rfr_id=info:sid/en.wikipedia.org:3alpha(or 20beta)-hydroxysteroid dehydrogenase" class="Z3988">
- Sweet F, Samant BR (1980). "Bifunctional Enzyme Activity at the Same Active Site: Study of 3α and 20β Activity by Affinity Alkylation of 3α,20β-Hydroxysteroid Dehydrogenase with 17-(Bromoacetoxy)steroids". Biochemistry. 19 (5): 978–86. doi:10.1021/bi00546a023. PMID 6928375.978-86&rft.date=1980&rft_id=info:doi/10.1021/bi00546a023&rft_id=info:pmid/6928375&rft.aulast=Sweet&rft.aufirst=F&rft.au=Samant, BR&rfr_id=info:sid/en.wikipedia.org:3alpha(or 20beta)-hydroxysteroid dehydrogenase" class="Z3988">
