FBRS
| FBRS | |||||||||||||||||||||||||
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| Identifikatori | |||||||||||||||||||||||||
| Aliasi | FBRS | ||||||||||||||||||||||||
| Vanjski ID-jevi | OMIM: 608601 MGI: 104648 HomoloGene: 79554 GeneCards: FBRS | ||||||||||||||||||||||||
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| Ortolozi | |||||||||||||||||||||||||
| Vrste | Čovjek | Miš | |||||||||||||||||||||||
| Entrez | |||||||||||||||||||||||||
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| RefSeq (mRNK) | |||||||||||||||||||||||||
| RefSeq (bjelančevina) | |||||||||||||||||||||||||
| Lokacija (UCSC) | Chr 16: 30.66 – 30.67 Mb | Chr 7: 127.08 – 127.09 Mb | |||||||||||||||||||||||
| PubMed pretraga | [3] | [4] | |||||||||||||||||||||||
| Wikipodaci | |||||||||||||||||||||||||
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Vjerovatni protein dugog transkripta fibrozin-1 je protein koji je kod ljudi kodiran genom FBRS.[5][6][7]
Fibrozin je limfokin kojeg luče aktivirani limfociti, a inducita proliferaciju fibroblasta (Prakash i Robbins, 1998)., prema OMIM-u.[7]
Aminokiselinska sekvenca
Dužina polipeptidnog lanca je 460 aminokiselina, a molekulska težina 48.388 Da.[8]
- Simboli
C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin
| 10 | 20 | 30 | 40 | 50 | ||||
|---|---|---|---|---|---|---|---|---|
| MFEKYPGKME | GLFRHNPYTA | FPPAVPGLPP | GLPPAVSFGS | LQGAFQPKST | ||||
| NPELPPRLGP | VPSGLSQKGT | QIPDHFRPPL | RKPGKWCAMH | VRVAYMILRH | ||||
| QEKMKGDSHK | LDFRNDLLPC | LPGPYGALPP | GQELSHPASL | FTATGAVHAA | ||||
| ANPFTAAPGA | HGPFLSPSTH | IDPFGRPTSF | ASLAALSNGA | FGGLGSPTFN | ||||
| SGAVFAQKES | PGAPPAFASP | PDPWGRLHRS | PLTFPAWVRP | PEAARTPGSD | ||||
| KERPVERREP | SITKEEKDRD | LPFSRPQLRV | SPATPKARAG | EEGPRPTKES | ||||
| VRVKEERKEE | AAAAAAAAAA | AAAAAAAAAT | GPQGLHLLFE | RPRPPPFLGP | ||||
| SPPDRCAGFL | EPTWLAAPPR | LARPPRFYEA | GEELTGPGAV | AAARLYGLEP | ||||
| AHPLLYSRLA | PPPPPAAAPG | TPHLLSKTPP | GALLGAPPPL | VPAPRPSSPP | ||||
| RGPGPARADR |
Kloniranje i ekspresija
[uredi | uredi izvor]Koristeći mišji fibrozin kao sondu za pregled biblioteka cDNK leukocita periferne krvi i posteljice, Prakash i Robbins (1998) klonirali su FBS1. Izvedeni protein sa 177 aminokiselina ima najmanje jednomjesto N-glikozilacije. Gel filtracija je pokazala da FBS1 ima prividnu molekulski masu od oko 25 kD. Mišji i ljudski fibrozin imaju 72,5% aminokiselinskog identiteta. Northern blot analizom u posteljici, otkriveni su transkripti od 4,4 kb i 2,0 do 2,4 kb. Ekspresija je pronađena i u skeletnim mišićima, gušterači, timusu, sjemenicima i leukocitima. Prakash, S., Robbins, P. W. Cloning and analysis of the cDNA for human fibrosin, a novel fibrogenic lymphokine. DNA Cell Biol. 17: 879-884, 1998. PubMed: 9809749</ref>
Funkcija gena
[uredi | uredi izvor]Prakash i Robbins (1998) pokazali su da se fibrozin izlučuje u medij za kulturu limfocitima stimuliranim mitogenom i indukuje proliferaciju u primarnim ljudskim diploidnim fibroblastima.
Reference
[uredi | uredi izvor]- ^ a b c GRCh38: Ensembl release 89: ENSG00000156860 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000042423 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Prakash S, Robbins PW, Wyler DJ (Apr 1995). "Cloning and analysis of murine cDNA that encodes a fibrogenic lymphokine, fibrosin". Proc Natl Acad Sci U S A. 92 (6): 2154–8. doi:10.1073/pnas.92.6.2154. PMC 42442. PMID 7892239.
- ^ Prakash S, Robbins PW (Nov 1998). "Cloning and analysis of the cDNA for human fibrosin, a novel fibrogenic lymphokine". DNA Cell Biol. 17 (10): 879–84. doi:10.1089/dna.1998.17.879. PMID 9809749.
- ^ a b "Entrez Gene: FBS1 fibrosin 1".
- ^ "UniProt, Q9HAH7". Pristupljeno 5. 7. 2021.
Dopunska literatura
[uredi | uredi izvor]- Hillier LD, Lennon G, Becker M, et al. (1997). "Generation and analysis of 280,000 human expressed sequence tags". Genome Res. 6 (9): 807–28. doi:10.1101/gr.6.9.807. PMID 8889549.
- Prakash S, Nanji AA, Robbins PW (1999). "Fibrosin: A novel lymphokine in alcohol-induced fibrosis". Exp. Mol. Pathol. 67 (1): 40–9. doi:10.1006/exmp.1999.2274. PMID 10493891.
- Prakash S, Robbins PW (2002). "Fibrosin: a novel lymphokine in wound healing". Exp. Mol. Pathol. 71 (3): 247–55. doi:10.1006/exmp.2001.2402. PMID 11733950.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
- Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
- Kimura K, Wakamatsu A, Suzuki Y, et al. (2006). "Diversification of transcriptional modulation: Large-scale identification and characterization of putative alternative promoters of human genes". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560.
- Prakash S, Paul WE, Robbins PW (2007). "Fibrosin, a novel fibrogenic cytokine, modulates expression of myofibroblasts". Exp. Mol. Pathol. 82 (1): 42–8. doi:10.1016/j.yexmp.2006.06.008. PMID 17083929.